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Image Search Results
Journal: Oncotarget
Article Title: TRIP-Br1 oncoprotein inhibits autophagy, apoptosis, and necroptosis under nutrient/serum-deprived condition
doi:
Figure Lengend Snippet: A. Autophagy, apoptosis, and necroptosis were checked in MCF7 and MDA-MB-231 cancer and MCF10A normal cell lines under the conditions of overcrowding or serum starvation. The cells were grown until either they became overcrowded with high cell densities or they were maintained in DMEM or DMEM/F12 media that did not contain FBS or horse serum for 24 or 48 hours. After the time periods indicated had elapsed, the cells were collected and subjected to Western blot analyses. γ-tubulin was used as a control marker. B. MCF7 and MDA-MB-231 cells were transfected with scrambled RNA (scRNA) or TRIP-Br1 silencing siRNA (siTRIP-Br1) and then incubated in media with or without serum for 24 or 48 hours. Western blot analysis was employed to determine the effect of TRIP-Br1 on autophagy, apoptosis, and necroptosis by means of corresponding biomarkers and correlated regulatory proteins. CM: normal condition with complete media; SS: serum-starved condition for 24 or 48 hours. The loading control was γ-tubulin.
Article Snippet: For serum-starvation conditions, cells were maintained in
Techniques: Western Blot, Marker, Transfection, Incubation